solid-phase sandwich enzyme-linked immunosorbent assay premier rotaclone Search Results


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quantikine® solid phase elisas  (R&D Systems Hematology)
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solid phase two site enzyme immunoassay (elisa  (Mercodia Inc)
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solid phase elisa assay  (R&D Systems)
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solid-phase elisa immunoassays  (Cold Spring Harbor Laboratory Meetings)
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solid-phase enzyme linked immunosorbent assay (elisa  (R&D Systems)
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solid-phase sandwich enzyme-linked immunosorbent assay  (ALPCO)
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solid phase enzyme-linked immunosorbent assay afp elisa assay  (DRG Instruments GmbH)
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immunosorbent assay kit  (Cell Signaling Technology Inc)
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solid phase sandwich elisa kits  (Cusabio)
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Cusabio solid phase sandwich elisa kits
Fig. 7 M1 microglia secrete Wnt5a, while M2c microglia secrete the Wnt7a protein. a OX-42 staining of microglial cells following 24 h of stimulation with polarization agents to obtain the M1, M2a, and M2c activation states. b, d Wnt5a and Wnt7a gene expression by microglia in different activation states after 6 and 24 h of polarization. c, e Wnt5a and Wnt7a protein secreted after 24 h of polarization, evaluated in the supernatant of the cultures by <t>ELISA.</t> N = 4 independent experiments with three replicates. Statistics: *p ≤0.05; **p ≤0.01; ***p ≤0.001 vs. Control; ANOVA followed by Bonferroni’s post-hoc test. Scale bar: 25 μm
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immunocomb® solid-phase elisa  (Biogal Labs)
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Biogal Labs immunocomb® solid-phase elisa
Fig. 7 M1 microglia secrete Wnt5a, while M2c microglia secrete the Wnt7a protein. a OX-42 staining of microglial cells following 24 h of stimulation with polarization agents to obtain the M1, M2a, and M2c activation states. b, d Wnt5a and Wnt7a gene expression by microglia in different activation states after 6 and 24 h of polarization. c, e Wnt5a and Wnt7a protein secreted after 24 h of polarization, evaluated in the supernatant of the cultures by <t>ELISA.</t> N = 4 independent experiments with three replicates. Statistics: *p ≤0.05; **p ≤0.01; ***p ≤0.001 vs. Control; ANOVA followed by Bonferroni’s post-hoc test. Scale bar: 25 μm
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Image Search Results


Fig. 7 M1 microglia secrete Wnt5a, while M2c microglia secrete the Wnt7a protein. a OX-42 staining of microglial cells following 24 h of stimulation with polarization agents to obtain the M1, M2a, and M2c activation states. b, d Wnt5a and Wnt7a gene expression by microglia in different activation states after 6 and 24 h of polarization. c, e Wnt5a and Wnt7a protein secreted after 24 h of polarization, evaluated in the supernatant of the cultures by ELISA. N = 4 independent experiments with three replicates. Statistics: *p ≤0.05; **p ≤0.01; ***p ≤0.001 vs. Control; ANOVA followed by Bonferroni’s post-hoc test. Scale bar: 25 μm

Journal: Journal of neuroinflammation

Article Title: Involvement of Wnt7a in the role of M2c microglia in neural stem cell oligodendrogenesis.

doi: 10.1186/s12974-020-01734-3

Figure Lengend Snippet: Fig. 7 M1 microglia secrete Wnt5a, while M2c microglia secrete the Wnt7a protein. a OX-42 staining of microglial cells following 24 h of stimulation with polarization agents to obtain the M1, M2a, and M2c activation states. b, d Wnt5a and Wnt7a gene expression by microglia in different activation states after 6 and 24 h of polarization. c, e Wnt5a and Wnt7a protein secreted after 24 h of polarization, evaluated in the supernatant of the cultures by ELISA. N = 4 independent experiments with three replicates. Statistics: *p ≤0.05; **p ≤0.01; ***p ≤0.001 vs. Control; ANOVA followed by Bonferroni’s post-hoc test. Scale bar: 25 μm

Article Snippet: The Wnt5a and Wnt7a in the supernatants of microglia cultures were measured using specific solid-phase sandwich ELISA kits (CSB-EL026138RA and CSB-EL026141MO, Cusabio) following the manufacturer’s recommendations (four independent experiments with three replicates were studied).

Techniques: Staining, Activation Assay, Gene Expression, Enzyme-linked Immunosorbent Assay, Control